Article
Restriction enzyme fragment length polymorphisms of amplified herpes simplex virus type-1 DNA provide epidemiologic information.
Diagnostic microbiology and infectious disease - 1 Jan 2000
Haugen T H, Alden B, Matthey S, Nicholson D
Abstract excerpt
Human herpes simplex type 1 (HSV-1) DNA of isolates from patients in a large teaching hospital was amplified by the polymerase chain reaction (PCR). The PCR products targeted approximately 2100 nt regions of relatively low G + C content. Comparison of restriction enzyme digests of amplified DNA s...
Topics
- Base Sequence
- Cytosine
- DNA Primers
- DNA, Viral
- Genetic Variation
- Guanine
- Herpes Simplex
- Herpesvirus 1, Human
- Humans
- Iowa
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length