Article
Transient expression of wild type and mutant human apolipoprotein AI in COS cells.
Biological chemistry Hoppe-Seyler - 1 Jul 1991
Stoffel W, Binczek E
Abstract excerpt
A human apolipoprotein AI (apo AI) minigene and two mutants were cloned into the vector pUHD10-1 for expression studies in COS cells under the control of the strong CMV (cytomegalovirus) enhancer and the own apo AI promoter. In the mutated apo AI minigene (mutant M1) the positions of the triplets...
Topics
- Amino Acid Sequence
- Animals
- Apolipoprotein A-I
- Cell Transformation, Neoplastic
- Cells, Cultured
- Chlorocebus aethiops
- Gene Expression
- Genes, Synthetic
- Humans
- Molecular Sequence Data
- Mutation
- Protein Biosynthesis
- Protein Conformation
- Sulfur Radioisotopes
- Transcription, Genetic