Article
Effectivity of expression of mature forms of mutant human apolipoprotein A-I.
Protein expression and purification - 1 Nov 1999
Sviridov D, Luong A, Pyle L, Fidge N
Abstract excerpt
In order to probe the structural and functional properties of a central region of apolipoprotein A-I (apoA-I), we engineered mutants of the mature form of the protein and expressed them using the baculovirus/insect cell expression system. The mutations which targeted the region of apoA-I between amino acids 140 and 150 included: (i) deletion of the region 140-150 (apoA-I(Delta140-150)); (ii) substitution of...
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