Article
An approach to the functional analysis of lecithin-cholesterol acyltransferase. Activation by recombinant normal and mutagenized apolipoprotein AI.
Biological chemistry Hoppe-Seyler - 1 Mar 1991
Bruhn H, Stoffel W
Abstract excerpt
Apolipoprotein AI (apo AI) of human serum high-density lipoprotein functions as an activator of lecithin-cholesterol acyltransferase (LCAT) and therefore plays an important role in reversed cholesterol transport. The mechanism of the acyltransfer, the activating polypeptide domains of apo AI and the active site of LCAT in this transesterification are not yet known. Synthetic peptides of the apo AI sequence have...
Topics
- Amino Acid Sequence
- Apolipoprotein A-I
- Apolipoproteins A
- Base Sequence
- Blotting, Western
- DNA, Bacterial
- Electrophoresis, Polyacrylamide Gel
- Enzyme Activation
- Escherichia coli
- Gene Expression Regulation, Bacterial