Article
Role of chitin binding domain of chitinase A of Streptomyces cyaneus SP-27 in protoplast formation from Schizophyllum commune.
Bioscience, biotechnology, and biochemistry - 23 Mar 2009
Yano Shigekazu, Honda Arata, Rattanakit-Chandet Nopakarn, Noda Yuta, Wakayama Mamoru, Plikomol Abhinya, Tachiki Takashi
Abstract excerpt
Chitinase A (CHIA) of Streptomyces cyaneus SP-27 forms protoplasts from Schizophyllum commune mycelia when it is combined with alpha-1,3-glucanase of Bacillus circulans KA-304. An N-terminal chitin-binding domain truncated mutant (CatCHIA), which was expressed in Escherichia coli Rosetta-gami B (DE 3), lost most of its colloidal chitin- and powder chitin-binding activity. The colloidal chitin-hydrolyzing, the...
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