Dispersed phantom scatterer technique reveals subtle differences in substrate recognition by phospholipase D inactive mutants.

Abstract excerpt

An elastic light-scattering-based method that makes use of phantom nanoparticles as a substrate organizer (see illustration) allowed the quantitative evaluation of the molecular recognition events between a series of inactivated mutants of phospholipase D and lysophosphatidylcholine. The results highlight the remarkable effects on binding capability caused by single amino acid substitutions.

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