Article
Intein-mediated protein assembly in transgenic wheat: production of active barnase and acetolactate synthase from split genes.
Plant biotechnology journal - 1 Apr 2009
Kempe Katja, Rubtsova Myroslava, Gils Mario
Abstract excerpt
Engineering traits by the assembly of non-functional gene products is a promising tool for modern plant biotechnology. In this article, we describe the establishment of male sterility and herbicide resistance in wheat (Triticum aestivum) by complementing inactive precursor protein fragments through a split intein system. N- and C-terminal fragments of a barnase gene from Bacillus amyloliquifaciens were fused to...
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