Article
Expression of antisense RNA against initiation factor eIF-4E mRNA in HeLa cells results in lengthened cell division times, diminished translation rates, and reduced levels of both eIF-4E and the p220 component of eIF-4F.
Molecular and cellular biology - 1 Nov 1991
De Benedetti A, Joshi-Barve S, Rinker-Schaeffer C, Rhoads R E
Abstract excerpt
HeLa cells were transformed to express antisense RNA against initiation factor eIF-4E mRNA from an inducible promoter. In the absence of inducer, these cells (AS cells) were morphologically similar to control cells but grew four- to sevenfold more slowly. Induction of antisense RNA production was lethal. Both eIF-4E mRNA and protein levels were reduced in proportion to the degree of antisense RNA expression, as...
Topics
- Actins
- Base Sequence
- Blotting, Northern
- Cell Division
- Eukaryotic Initiation Factor-4E
- Eukaryotic Initiation Factor-4F
- Genetic Vectors
- HeLa Cells
- Humans
- Kinetics