Article
Massively parallel sequencing identifies the gene Megf8 with ENU-induced mutation causing heterotaxy.
Proceedings of the National Academy of Sciences of the United States of America - 3 Mar 2009
Zhang Zhen, Alpert Deanne, Francis Richard, Chatterjee Bishwanath, Yu Qing, Tansey Terry, Sabol Steven L, Cui Cheng, Bai Yongli, Koriabine Maxim, Yoshinaga Yuko, Cheng Jan-Fang, Chen Feng, Martin Joel, Schackwitz Wendy, Gunn Teresa M, Kramer Kenneth L, De Jong Pieter J, Pennacchio Len A, Lo Cecilia W
Abstract excerpt
Forward genetic screens with ENU (N-ethyl-N-nitrosourea) mutagenesis can facilitate gene discovery, but mutation identification is often difficult. We present the first study in which an ENU-induced mutation was identified by massively parallel DNA sequencing. This mutation causes heterotaxy and complex congenital heart defects and was mapped to a 2.2-Mb interval on mouse chromosome 7. Massively parallel...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.