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Article

The 5' end of coronavirus minus-strand RNAs contains a short poly(U) tract

1 Nov 1991

Abstract excerpt

A radiolabeled oligodeoxynucleotide primer that anneals near the common 5' end of bovine coronavirus minus-strand RNAs was extended with reverse transcriptase, and a major product suggesting poly(U) tracts of 8 to 20 nucleotides was found. The extended primer molecules were ligated head to tail, amplified by the polymerase chain reaction, cloned, and sequenced, and poly(U) tracts of 9 to 26 nucleotides were...

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Identifiers

PMID: 1920635
DOI: 10.1128/jvi.65.11.6331-6333.1991

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