Allele-specific PCR assay to genotype SNP rs7903146 in TCF7L2 gene for rapid screening of diabetes susceptibility.

doi:10.1590/s0004-27302008000800026

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Allele-specific PCR assay to genotype SNP rs7903146 in TCF7L2 gene for rapid screening of diabetes susceptibility.

Arquivos brasileiros de endocrinologia e metabologia - 1 Nov 2008

Dutra Ludmila Alves Sanches, Costa Patrícia Godoy Garcia, Velasco Lara Franciele Ribeiro, Amato Angélica Amorim, Barra Gustavo Barcelos

Abstract excerpt

OBJECTIVE: The aim of the present study is to validate a rapid and simple allele-specific PCR that genotypes TCF7/L2 rs7903146 (C/T) polymorphism with standard PCR instruments. METHODS: Two forward primers with variations in their 3' nucleotides were designed in such a way that each was specific for one of the two variants. They were combined with a common reverse primer into two PCR reactions. Specific...

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PMID: 19169495
DOI: 10.1590/s0004-27302008000800026

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

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