Article
Dual-color microchip electrophoresis with single-photon avalanche diodes: application to mutation detection.
Electrophoresis - 1 Dec 2008
Stenirri Stefania, Cretich Marina, Rech Ivan, Restelli Alessandro, Ghioni Massimo, Cova Sergio, Ferrari Maurizio, Cremonesi Laura, Chiari Marcella
Abstract excerpt
A novel microchip electrophoresis instrument based on single-photon avalanche diodes was used for the molecular characterization of mutations in disease genes. The identification of the main mutation causing cystic fibrosis, named DeltaF508, by the Amplification Refractory Mutation System was used to validate the technology. In our implemented protocol the wild-type and mutant allele-specific primers are labeled...
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