Article
The mutation Lys234His yields a class A beta-lactamase with a novel pH-dependence.
The Biochemical journal - 15 Sept 1991
Brannigan J, Matagne A, Jacob F, Damblon C, Joris B, Klein D, Spratt B G, Frère J M
Abstract excerpt
The lysine-234 residue is highly conserved in beta-lactamases and in nearly all active-site-serine penicillin-recognizing enzymes. Its replacement by a histidine residue in the Streptomyces albus G class A beta-lactamase yielded an enzyme the pH-dependence of which was characterized by the appearance of a novel pK, which could be attributed to the newly introduced residue. At low pH, the kcat, value for...
Topics
- Ampicillin
- Base Sequence
- Binding Sites
- Enzyme Stability
- Gene Expression
- Histidine
- Hot Temperature
- Hydrogen-Ion Concentration
- Hydrolysis
- Kinetics
- Lysine