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Article

The mutation Lys234His yields a class A beta-lactamase with a novel pH-dependence.

The Biochemical journal - 15 Sept 1991

Brannigan J, Matagne A, Jacob F, Damblon C, Joris B, Klein D, Spratt B G, Frère J M

Abstract excerpt

The lysine-234 residue is highly conserved in beta-lactamases and in nearly all active-site-serine penicillin-recognizing enzymes. Its replacement by a histidine residue in the Streptomyces albus G class A beta-lactamase yielded an enzyme the pH-dependence of which was characterized by the appearance of a novel pK, which could be attributed to the newly introduced residue. At low pH, the kcat, value for...

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Identifiers

PMID: 1910335
DOI: 10.1042/bj2780673
PMCID: PMC1151399

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Source receipt: pubmed, retrieved 7 Sept 2026.

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