Article
T-DNA activation tagging as a tool to isolate regulators of a metabolic pathway from a genetically non-tractable plant species.
Transgenic research - 1 Dec 2001
van der Fits L, Hilliou F, Memelink J
Abstract excerpt
T-DNA activation tagging is a method used to generate dominant mutations in plants or plant cells by the insertion of a T-DNA which carries constitutive enhancer elements that can cause transcriptional activation of flanking plant genes. We applied this approach to the species Catharanthus roseus (L.) G. Don (Madagascar periwinkle), in an attempt to isolate regulators of genes that are involved in the...
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