Cytopathogenic effects in enterocytelike Caco-2 cells differentiate virulent from avirulent Listeria strains.

Abstract excerpt

We have developed a simple test that differentiates between virulent and avirulent Listeria species as defined by the mouse 50% lethal doses (LD50S). The assay is based on trypan blue-revealed cytopathogenic effects that are produced during the infection of the human enterocytelike cell line Caco-2. These effects were elicited only by Listeria strains that had an intraperitoneal mouse LD50 less than 10(8) and...

Topics

• Animals
• Bacteriological Techniques
• Cell Line
• Evaluation Studies as Topic
• Humans
• Lethal Dose 50
• Listeria
• Listeria monocytogenes
• Mice
• Mutation
• Trypan Blue