Article
Identification of specific amphipathic alpha-helical sequence of human apolipoprotein A-IV involved in lecithin:cholesterol acyltransferase activation.
The Journal of biological chemistry - 25 Nov 1994
Emmanuel F, Steinmetz A, Rosseneu M, Brasseur R, Gosselet N, Attenot F, Cuiné S, Séguret S, Latta M, Fruchart J C
Abstract excerpt
To investigate the structure-function relationship of human apolipoprotein A-IV (apoA-IV), several deletion mutants of this protein were constructed by sequentially removing pairs of 22-residue repeats, potentially having an amphipathic alpha-helical conformation. The mutants, produced as recombi...
Topics
- Amino Acid Sequence
- Apolipoproteins A
- Dimyristoylphosphatidylcholine
- Electrophoresis, Polyacrylamide Gel
- Enzyme Activation
- Escherichia coli
- Humans
- Microscopy, Electron
- Molecular Sequence Data
- Mutation
- Phosphatidylcholine-Sterol O-Acyltransferase
- Protein Conformation
- Sequence Alignment