Article
Characterization of the rpoC gene of Streptomyces coelicolor A3(2) and its use to develop a simple and rapid method for the purification of RNA polymerase.
Gene - 1 Sept 1997
Babcock M J, Buttner M J, Keler C H, Clarke B R, Morris R A, Lewis C G, Brawner M E
Abstract excerpt
The Streptomyces coelicolor rpoC gene, that encodes the beta' subunit of RNA polymerase, was isolated using the Escherichia coli rpoC gene as a hybridization probe. Comparison of the predicted amino acid sequence of the S. coelicolor beta' subunit to those characterized from other bacteria reveal...
Topics
- Amino Acid Sequence
- Base Sequence
- Chromatography, Affinity
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA-Directed RNA Polymerases
- Histidine
- Molecular Sequence Data
- Mutation
- Nickel
- Plant Proteins
- Recombinant Proteins
- Recombination, Genetic
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Streptomyces