Article
A novel method for determining functional LDL receptor activity in familial hypercholesterolemia: application of the CD3/CD28 assay in lymphocytes.
Clinica chimica acta; international journal of clinical chemistry - 1 Feb 2009
Tada Hayato, Kawashiri Masa-aki, Noguchi Tohru, Mori Mika, Tsuchida Masayuki, Takata Mutsuko, Nohara Atsushi, Inazu Akihiro, Kobayashi Junji, Yachie Akihiro, Mabuchi Hiroshi, Yamagishi Masakazu
Abstract excerpt
BACKGROUND: The objective of this study was to develop a new and simple method for measuring low-density lipoprotein receptor (LDLR) activity using peripheral lymphocytes enabling us to clinically diagnose familial hypercholesterolemia (FH) and ascertain the involved mutations (such as K790X mutation), that might not be clearly detected in the conventional method. METHODS: Our method comprised the following 2...
Topics
- Alleles
- Animals
- Antibodies
- Blood Chemical Analysis
- CD28 Antigens
- CD3 Complex
- Female
- Heparin
- Heterozygote
- Humans
- Hyperlipoproteinemia Type II
- Lipoproteins
- Lymphocytes