Article
Flow cytometry with a monoclonal antibody to the low density lipoprotein receptor compared with gene mutation detection in diagnosis of heterozygous familial hypercholesterolemia.
Clinical chemistry - 1 May 1998
Raungaard B, Heath F, Brorholt-Petersen J U, Jensen H K, Faergeman O
Abstract excerpt
We used a fluorescence flow cytometry assay with a monoclonal low density lipoprotein (LDL) receptor-specific antibody to detect LDL receptor expression on blood T lymphocytes and monocytes. We prepared peripheral blood mononuclear cells from patients with genetically verified LDL receptor-defect...
Topics
- Amino Acid Substitution
- Antibodies, Monoclonal
- Cell Count
- DNA Mutational Analysis
- Flow Cytometry
- Heterozygote
- Humans
- Hyperlipoproteinemia Type II
- Lymphocyte Activation
- Monocytes
- Mutation