Article
Electrothermal atomic absorption spectrometric diagnosis of familial hypercholesterolemia.
Analytical chemistry - 1 Jun 2000
Martín de Llano J J, Andreu E J, Pastor A, de la Guardia M, Knecht E
Abstract excerpt
We have developed a new nonradioactive assay to identify human low-density lipoprotein receptor defects. It is based on the incubation of cultured cells with colloidal gold-LDL conjugates and quantitation of the gold associated with the cells by electrothermal atomic absorption spectrometry. After an oxidative treatment with nitric and hydrochloric acids, the biological matrix interferes neither with the gold...
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