Article
The biotin-capture lipid affinity assay: a rapid method for determining lipid binding parameters for apolipoproteins.
Journal of lipid research - 1 Feb 2006
Davidson W Sean, Ghering Amy B, Beish Lauren, Tubb Matthew R, Hui David Y, Pearson Kevin
Abstract excerpt
The lipid affinity of plasma apolipoproteins is an important modulator of lipoprotein metabolism. Mutagenesis techniques have been widely used to modulate apolipoprotein lipid affinity for studying biological function, but the approach requires rapid and reliable lipid affinity assays to compare the mutants. Here, we describe a novel method that measures apolipoprotein binding to a standardized preparation of...
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