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Article

Locked nucleic acid pentamers as universal PCR primers for genomic DNA amplification.

PloS one - 1 Jan 2008

Sun Zhen, Chen Zhi, Hou Xiaoli, Li Shuping, Zhu Haihong, Qian Ji, Lu Daru, Liu Wei

Abstract excerpt

BACKGROUND: Multiplexing technologies, which allow for simultaneous detection of multiple nucleic acid sequences in a single reaction, can save a lot of time, cost and labor compared to traditional single reaction detection methods. However, the multiplexing method currently used requires precise handiwork and many complicated steps, making a new, simpler technique desirable. Oligonucleotides containing locked...

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PMID: 19002243
DOI: 10.1371/journal.pone.0003701
PMCID: PMC2577006

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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