Article
Hydrogen bond network of cytochrome P-450cam: a network connecting the heme group with helix K.
Biochimica et biophysica acta - 13 Jul 1992
Yoshikawa K, Noguti T, Tsujimura M, Koga H, Yasukochi T, Horiuchi T, Go M
Abstract excerpt
During investigations of the structural character of a mutant P-450cam where Glu-286 is replaced with lysine, we obtained evidence of a hydrogen bond network between helix K and the heme group via helix L of P-450cam. This mutant protein loses the ability to maintain the heme group in a proper position, possibly due to a break in the hydrogen bond network.
Topics
- Arginine
- Cytochrome P-450 Enzyme System
- Heme
- Hydrogen Bonding
- Lysine
- Models, Molecular
- Mutation
- Protein Conformation