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[In vitro cross-linking of Escherichia coli tartrate dehydratase beta subunit].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Aug 2008

Xu Yan, Gao Yin

Abstract excerpt

To test the hypothesis that in vitro protein cross-linking could be accomplished in three concerted steps: (1) a change in protein conformation; (2) formation of interchain disulfide bonds; and (3) formation of interchain isopeptide cross-links, we amplified wild and Cys/Ser mutant genes with PCR technique from E. coli BL21 cells and subcloned them into expression plasmid pTrcHisC. Recombinant proteins, which...

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PMID: 18998556

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SDS

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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