Article
Extracellular protons regulate human ENaC by modulating Na+ self-inhibition.
The Journal of biological chemistry - 9 Jan 2009
Collier Daniel M, Snyder Peter M
Abstract excerpt
The epithelial Na(+) channel, ENaC, is exposed to a wide range of proton concentrations in the kidney, lung, and sweat duct. We, therefore, tested whether pH alters ENaC activity. In Xenopus oocytes expressing human alpha-, beta-, and gammaENaC, amiloride-sensitive current was altered by protons in the physiologically relevant range (pH 8.5-6.0). Compared with pH 7.4, acidic pH increased ENaC current, whereas...
Topics
- Animals
- Cations
- Cells, Cultured
- Electrophysiology
- Epithelial Sodium Channels
- Female
- Humans
- Hydrogen-Ion Concentration
- Ion Channel Gating
- Mutation
- Patch-Clamp Techniques
- Protons