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Article

Engineered humanized diabodies for microPET imaging of prostate stem cell antigen-expressing tumors.

Protein engineering, design & selection : PEDS - 1 Mar 2009

Leyton Jeffrey V, Olafsen Tove, Sherman Mark A, Bauer Karl B, Aghajanian Patrick, Reiter Robert E, Wu Anna M

Abstract excerpt

We have previously demonstrated preclinical in vivo targeting of prostate stem cell antigen (PSCA) using a humanized anti-PSCA 2B3 monoclonal antibody (mAb). However, humanization resulted in 5-fold loss of apparent affinity relative to the parental mAb (1 nM). In this study, diabodies (scFv dimers of 55 kDa) were generated from 2B3 including variants with different linker lengths as well as back-mutations to...

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Identifiers

PMID: 18957406
DOI: 10.1093/protein/gzn055
PMCID: PMC2644405

Genes

PSCA

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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