Article
Detection of endogenous Snf1 and its activation state: application to Saccharomyces and Candida species.
Yeast (Chichester, England) - 1 Oct 2008
Orlova Marianna, Barrett LaKisha, Kuchin Sergei
Abstract excerpt
The stress-response Snf1 protein kinase of Saccharomyces cerevisiae serves as a powerful model for studies of the eukaryotic Snf1/AMP-activated protein kinase (AMPK) family. Central to studies of Snf1 are methods that determine its activation state under various physiological and genetic conditions. Here, we have developed a convenient and sensitive method for immunoblot analysis of endogenous yeast Snf1 and its...
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