Detection of endogenous Snf1 and its activation state: application to Saccharomyces and Candida species.

doi:10.1002/yea.1628

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Article

Detection of endogenous Snf1 and its activation state: application to Saccharomyces and Candida species.

Yeast (Chichester, England) - 1 Oct 2008

Orlova Marianna, Barrett LaKisha, Kuchin Sergei

Abstract excerpt

The stress-response Snf1 protein kinase of Saccharomyces cerevisiae serves as a powerful model for studies of the eukaryotic Snf1/AMP-activated protein kinase (AMPK) family. Central to studies of Snf1 are methods that determine its activation state under various physiological and genetic conditions. Here, we have developed a convenient and sensitive method for immunoblot analysis of endogenous yeast Snf1 and its...

Topics

Candida
Enzyme Activation
Gene Expression Regulation, Fungal
Immunoblotting
Mutation
Phosphorylation
Protein Serine-Threonine Kinases
Saccharomyces
Sensitivity and Specificity
Signal Transduction

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Open in PubMed

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PMID: 18949820
DOI: 10.1002/yea.1628

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