Article
Genotypic antiretroviral resistance testing for human immunodeficiency virus type 1 integrase inhibitors by use of the TruGene sequencing system.
Journal of clinical microbiology - 1 Dec 2008
Paar Christian, Palmetshofer Christina, Flieger Karin, Geit Maria, Kaiser Rolf, Stekel Herbert, Berg Jörg
Abstract excerpt
A sequencing assay for detection of mutations conferring resistance to human immunodeficiency virus type 1 (HIV-1) integrase inhibitors raltegravir and elvitegravir was developed using the automated TruGene sequencing system. The assay returned clear sequencing results for samples with >or=500 RNA copies/ml for mutation detection and HIV-1 subtyping across a spectrum of HIV-1 subtypes.
Topics
- Amino Acid Substitution
- Genotype
- HIV-1
- Humans
- Integrase Inhibitors
- Microbial Sensitivity Tests
- Mutation, Missense
- RNA, Viral
- Sequence Analysis, DNA