Article
Demonstration of murine pancreas elastase and its interstrain variation by isoelectric focusing.
Electrophoresis - 1 Jun 1991
von Deimling O H, Gaa A, Hameister H
Abstract excerpt
Isoelectric focusing between pH 9 and 11 was used for separation of murine pancreas proteases. One of these proteases is characterized by its preference for N-acetyl-L-alanine-alpha-naphthylester as substrate and by its genetic linkage to bt, a coat color marker of chromosome 15. This protease was identified as elastase, and is probably elastase-1 (ELA-1). Because of the simple procedure and the excellent...
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