Article
Hematopoiesis on suspended nylon screen-stromal cell microenvironments.
Journal of biomechanical engineering - 1 May 1991
Naughton B A, Tjota A, Sibanda B, Naughton G K
Abstract excerpt
A three-dimensional culture system for the growth of primate and rodent bone marrow was developed in our laboratory. This method involves the seeding of stromal cells onto a nylon screen and the inoculation of fresh or cryopreserved bone marrow hematopoietic cells after stromal cell processes had extended across 3 to 4 out of every 5 mesh openings. Stromal cells attach, grow, and secrete matrix proteins which...
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