Hematopoiesis on suspended nylon screen-stromal cell microenvironments.

doi:10.1115/1.2891230

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Article

Hematopoiesis on suspended nylon screen-stromal cell microenvironments.

Journal of biomechanical engineering - 1 May 1991

Naughton B A, Tjota A, Sibanda B, Naughton G K

Abstract excerpt

A three-dimensional culture system for the growth of primate and rodent bone marrow was developed in our laboratory. This method involves the seeding of stromal cells onto a nylon screen and the inoculation of fresh or cryopreserved bone marrow hematopoietic cells after stromal cell processes had extended across 3 to 4 out of every 5 mesh openings. Stromal cells attach, grow, and secrete matrix proteins which...

Topics

Animals
Antibodies, Monoclonal
Bone Marrow
Bone Marrow Cells
Cells, Cultured
Clone Cells
Colony-Forming Units Assay
Cyclophosphamide
Cytarabine
Cytotoxicity Tests, Immunologic
Dose-Response Relationship, Drug

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Flow Cytometry

Fluorouracil

Killer Cells, Natural

Male

Methotrexate

Phenotype

Rats

Source

Open in PubMed

Identifiers

PMID: 1875689
DOI: 10.1115/1.2891230

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1875689-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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