Article
High throughput automated allele frequency estimation by pyrosequencing.
PloS one - 16 Jul 2008
Doostzadeh Julie, Shokralla Shadi, Absalan Farnaz, Jalili Roxana, Mohandessi Sharareh, Langston James W, Davis Ronald W, Ronaghi Mostafa, Gharizadeh Baback
Abstract excerpt
Pyrosequencing is a DNA sequencing method based on the principle of sequencing-by-synthesis and pyrophosphate detection through a series of enzymatic reactions. This bioluminometric, real-time DNA sequencing technique offers unique applications that are cost-effective and user-friendly. In this study, we have combined a number of methods to develop an accurate, robust and cost efficient method to determine allele...
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