Article
Cloning, sequencing, and mutational analysis of the Bradyrhizobium japonicum fumC-like gene: evidence for the existence of two different fumarases.
Journal of general microbiology - 1 Apr 1991
Acuña G, Ebeling S, Hennecke H
Abstract excerpt
The Bradyrhizobium japonicum fumarase gene (fumC-like) was cloned and sequenced, and a fumC deletion mutant was constructed. This mutant had a Nod+ Fix+ phenotype in symbiosis with the host plant, soybean, and growth in minimal medium with fumarate as sole carbon source was also not affected. The cloned B. japonicum fumC gene fully complemented an Escherichia coli Fum- mutant, strain JH400, for growth in minimal...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Fumarate Hydratase
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Phenotype
- Restriction Mapping
- Rhizobiaceae