Article
Discovery and sequence analysis of bacterial genes involved in the biogenesis of c-type cytochromes.
The Journal of biological chemistry - 25 Apr 1991
Ramseier T M, Winteler H V, Hennecke H
Abstract excerpt
We report the DNA sequence and mutational analysis of a novel cluster of six Bradyrhizobium japonicum genes of which at least three (designated cycV, cycW, and cycX) are essential for the formation of all cellular c-type cytochromes. Mutants having insertions in these genes were completely devoid of any soluble (periplasmic) or membrane-bound c-type cytochromes; even the apo form of cytochrome c1 was not...
Topics
- Adenosine Triphosphate
- Amino Acid Sequence
- Base Sequence
- Blotting, Western
- Cytochrome c Group
- DNA, Bacterial
- Electrophoresis, Polyacrylamide Gel
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Open Reading Frames