Tn5 mutagenesis and insertion replacement in Azotobacter vinelandii.

doi:10.1016/0147-619x(91)90009-l

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Article

Tn5 mutagenesis and insertion replacement in Azotobacter vinelandii.

Plasmid - 1 Jan 1991

Contreras A, Maldonado R, Casadesus J

Abstract excerpt

Tn5 insertion mutants of Azotobacter vinelandii were isolated using vectors pJB4JI (IncP) and pGS9 (IncN). A procedure to replace Tn5 (Kmr) by its nontransposing derivative Tn5-131 (Tcr) was developed. For the replacement, a ColEl derivative harboring Tn5-131 (pRZ131) was conjugally mobilized by...

Topics

Azotobacter
Conjugation, Genetic
Crosses, Genetic
DNA Transposable Elements
Escherichia coli
Genetic Linkage
Mutagenesis, Insertional
Phenotype
Plasmids
Recombination, Genetic
Restriction Mapping

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