Article
Validation of a Tn5 transposon mutagenesis system for Gluconacetobacter diazotrophicus through characterization of a flagellar mutant.
Archives of microbiology - 1 Apr 2008
Rouws Luc F M, Simões-Araújo Jean L, Hemerly Adriana S, Baldani José I
Abstract excerpt
Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium, which was originally isolated from the interior of sugarcane plants. The genome of strain PAL5 of G. diazotrophicus has been completely sequenced and a next step is the functional characterization of its genes. The aim of this study was to establish an efficient mutagenesis method, using the commercial Tn5 transposon EZ::Tn5<KAN-2>Tnp Transposome...
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