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Article

Introduction of cytochrome b mutations in Saccharomyces cerevisiae by a method that allows selection for both functional and non-functional cytochrome b proteins.

Biochimica et biophysica acta - 1 Sept 2008

Ding Martina G, Butler Christine A, Saracco Scott A, Fox Thomas D, Godard François, di Rago Jean-Paul, Trumpower Bernard L

Abstract excerpt

We have previously used inhibitors interacting with the Qn site of the yeast cytochrome bc(1) complex to obtain yeast strains with resistance-conferring mutations in cytochrome b as a means to investigate the effects of amino acid substitutions on Qn site enzymatic activity [M.G. Ding, J.-P. di Rago, B.L. Trumpower, Investigating the Qn site of the cytochrome bc1 complex in Saccharomyces cerevisiae with mutants...

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Identifiers

PMID: 18498758
DOI: 10.1016/j.bbabio.2008.04.029
PMCID: PMC2601669

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