Article
Virulence-associated chromosomal loci of Shigella flexneri identified by random Tn5 insertion mutagenesis.
Molecular microbiology - 1 Jan 1991
Okada N, Sasakawa C, Tobe T, Yamada M, Nagai S, Talukder K A, Komatsu K, Kanegasaki S, Yoshikawa M
Abstract excerpt
Shigellae are the causative agents of bacillary dysentery and are capable of invading epithelial cells, multiplying therein and spreading into adjacent cells. To identify genes on the chromosome associated with the virulence phenotype, 9114 independent Tn5 insertion mutants were isolated in a virulent strain of Shigella flexneri. By using an in vitro assay for intercellular spread or an animal infection model,...
Topics
- Animals
- Antigens, Bacterial
- Blotting, Western
- Cells, Cultured
- Chromosomes, Bacterial
- DNA Transposable Elements
- Deoxyribonucleases, Type II Site-Specific
- Kinetics
- Lipopolysaccharides
- Mutagenesis, Insertional