Article
Probing the bottom of a folding funnel using conformationally gated electron transfer reactions.
Journal of the American Chemical Society - 18 Jun 2008
Bandi Swati, Bowler Bruce E
Abstract excerpt
The effect of global stability on the kinetics of interconversion between the native (N) and a compact, partially unfolded form (I) of iso-1-cytochrome c stabilized by His73-heme ligation is investigated using a novel conformationally gated ET method. For the K73H variant and the 2-fold less stable AcH73 variant, the N and I conformers are of nearly equal stability at pH 7.5. The pH jump kinetic data yield kobs =...
Topics
- Cytochromes c
- Electron Transport
- Heme
- Histidine
- Hydrogen-Ion Concentration
- Kinetics
- Ligands
- Mutation
- Protein Conformation
- Protein Folding