Article
Probing the dynamics of a His73-heme alkaline transition in a destabilized variant of yeast iso-1-cytochrome c with conformationally gated electron transfer methods.
Biochemistry - 22 Nov 2011
Bandi Swati, Bowler Bruce E
Abstract excerpt
The alkaline transition of cytochrome c involves substitution of the Met80 heme ligand of the native state with a lysine ligand from a surface Ω-loop (residues 70 to 85). The standard mechanism for the alkaline transition involves a rapid deprotonation equilibrium followed by the conformational change. However, recent work implicates multiple ionization equilibria and stable intermediates. In previous work, we...
Topics
- Cytochromes c1
- Electron Transport
- Heme
- Histidine
- Hydrogen-Ion Concentration
- Models, Molecular
- Mutation
- Oxidation-Reduction
- Protein Conformation
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins