Dynamics of the His79-heme alkaline transition of yeast iso-1-cytochrome c probed by conformationally gated electron transfer with Co(II)bis(terpyridine).

Abstract excerpt

Alkaline conformers of cytochrome c may be involved in both its electron transport and apoptotic functions. We use cobalt(II)bis(terpyridine), Co(terpy)2(2+), as a reagent for conformationally gated electron-transfer (gated ET) experiments to study the alkaline conformational transition of K79H variants of yeast iso-1-cytochrome c expressed in Escherichia coli , WT*K79H, with alanine at position 72 and...

Topics

• Alkalies
• Cytochromes c1
• Electron Transport
• Heme
• Histidine
• Hydrogen-Ion Concentration
• Models, Molecular
• Mutation
• Organometallic Compounds
• Saccharomyces cerevisiae