Article
Analysis of an Escherichia coli dnaB temperature-sensitive insertion mutation and its cold-sensitive extragenic suppressor.
The Journal of biological chemistry - 25 Feb 1991
Chang S F, Ng D, Baird L, Georgopoulos C
Abstract excerpt
An Escherichia coli mutant, ts121, was isolated following random insertional mutagenesis using phage lambda Mu transposition. The mutant phenotype includes inability to form colonies at temperatures above 38 degrees C and inability to propagate phage lambda at all temperatures. A lambda i434 cI-...
Topics
- Bacterial Proteins
- Base Sequence
- Blotting, Southern
- Blotting, Western
- Cold Temperature
- DNA Transposable Elements
- DNA, Bacterial
- Escherichia coli
- Gene Amplification
- Genes, Bacterial
- Genes, Suppressor
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Open Reading Frames
- Phenotype
- Plasmids
- Restriction Mapping
- Temperature