Article
Identification of side population cells in human hepatocellular carcinoma cell lines with stepwise metastatic potentials.
Journal of cancer research and clinical oncology - 1 Nov 2008
Shi Guo-Ming, Xu Yang, Fan Jia, Zhou Jian, Yang Xin-Rong, Qiu Shuang-Jian, Liao Yong, Wu Wei-Zhong, Ji Yuan, Ke Ai-Wu, Ding Zhen-Bin, He Yi-Zhou, Wu Bing, Yang Guo-Huan, Qin Wen-Zhen, Zhang Wu, Zhu Jiang, Min Zhi-Hui, Wu Zhi-Quan
Abstract excerpt
PURPOSE: To identify the side population (SP) cells from four hepatocellular carcinoma (HCC) cell lines with stepwise metastatic potentials. METHODS: SP cells were sorted from HCCLM3, MHCC97-H, MHCC97-L and Hep3B by flow cytometry, and then analyzed by differentiation study, clonogenic assay, chemoresistance study and tumorigenicity assay in vivo. The expression of ABCG(2) in SP cells was detected by...
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