Article
Increased mitochondrial thioredoxin 2 potentiates N-ethylmaleimide-induced cytotoxicity.
Chemical research in toxicology - 1 Jun 2008
Chen Yan, Go Young-Mi, Pohl Jan, Reed Matthew, Cai Jiyang, Jones Dean P
Abstract excerpt
Thioredoxin 2 (Trx2) is a mitochondrially localized antioxidant and antiapoptotic protein, whose functions are mainly dependent on the conserved cysteines at its redox active center. In the current study, we showed by mass spectrometry that a thiol alkylating agent, N-ethylmaleimide (NEM), alkylated a single cysteine residue in the active center of Trx2. The interaction between NEM and Trx2 in intact cells was...
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