Article
Embryonic chicken lens cells cultured in reconstituted basement membrane: an experimental model to maintain the epithelial phenotype in culture.
Growth regulation - 1 Jun 1991
Alemany J, Peralta Soler A, Smith R M, Roth J, Jarett L, de Pablo F
Abstract excerpt
The action of a reconstituted basement membrane has been studied on primary cultures of embryonic lens cells. When a solution of this matrix (Matrigel) was included in the culture medium, a high percentage of cells maintained the epithelial phenotype, judged by electron microscopy criteria, in contrast to the differentiated state induced by serum. Complete matrix stimulated by 6-fold the incorporation of...
Topics
- Animals
- Cells, Cultured
- Chick Embryo
- Collagen
- Drug Combinations
- Epithelial Cells
- Laminin
- Lens, Crystalline
- Mitosis
- Models, Biological
- Phenotype