Article
[Identification of a novel antigenic epitope on GST fusion-expressed and ORF2-encoded proteins of hepatitis E virus].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology - 1 Apr 2008
Liang Jiu-hong, Liang Li-min, Dong Min, Han Zhen-ge, Dong Chen, Meng Ji-hong
Abstract excerpt
AIM: To investigate the effect of a GST tag on the antigenic structure of GST fusion-expressed and ORF2-encoded recombinant proteins of hepatitis E virus (HEV). METHODS: The monoclonal antibodies (mAb) were prepared with a GST fusion protein, p166Chn-GST, which was derived from a Chinese HEV strain. Then they were tested by indirect ELISA, competition ELISA and Western blot with different GST fusion, His fusion...
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