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Article

Design of a multiplex nested PCR for genotyping of the NSP4 from group A rotavirus.

Journal of virological methods - 1 May 2008

Rodríguez-Díaz Jesús, Rubilar-Abreu Elba, Spitzner Markus, Hedlund Kjell-Olof, Liprandi Ferdinando, Svensson Lennart

Abstract excerpt

A novel PCR method was developed to discriminate amongst genotypes A-C of the rotavirus non-structural protein 4 (NSP4). Genotype-specific primers were designed that correctly identified the NSP4 genotype when evaluated as a multiplex PCR with cell culture adapted rotavirus strains. Rotavirus strains B223 SGIG6P6[1], NCDV SGIG6P6[1] and SA11 SGIG3P5B[2] were used as control for NSP4 genotype A; A34 SGIG5P14[23],...

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Identifiers

PMID: 18353449
DOI: 10.1016/j.jviromet.2008.01.030

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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