Article
Determination of human rotavirus VP6 genogroups I and II by reverse transcription-PCR.
Journal of clinical microbiology - 1 Oct 2008
Lin Yi-Pei, Kao Chuan-Liang, Chang Sui-Yuan, Taniguchi Koki, Hung Pei-Yung, Lin Hsueh-Ching, Huang Li-Min, Huang Hsueh-Hung, Yang Jyh-Yuan, Lee Chun-Nan
Abstract excerpt
Based on nucleotide sequence and phylogenetic analysis of the partial VP6 genes, group A rotaviruses can be mainly differentiated into two genogroups. In this study, a method employing reverse transcription-PCR (RT-PCR) and degenerate primers was established to assign the VP6 genogroup. VP6 genogroup I and genogroup II could be determined according to the sizes of the amplicons: 380 and 780 bp, respectively. The...
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