Article
Isolation and characterization of a Chlamydomonas reinhardtii mutant lacking the gamma-subunit of chloroplast coupling factor 1 (CF1).
Molecular and cellular biology - 1 Oct 1991
Smart E J, Selman B R
Abstract excerpt
Several nonphotoautotrophic mutants of Chlamydomonas reinhardtii were generated by transforming strain nit1-305 (cw 15) with exogenous DNA. An enrichment for potential photophosphorylation mutants was performed on medium containing arsenate, and acetate-requiring auxotrophs were then identified by replica plating. Strains containing a potential mutation in the nuclear DNA encoding the chloroplast coupling factor...
Topics
- Acetates
- Animals
- Arsenates
- Blotting, Northern
- Blotting, Southern
- Chlamydomonas
- Chloroplasts
- Chromosome Deletion
- Immunoblotting
- Mutation
- Polymerase Chain Reaction