Article
A single amino acid substitution changes the substrate specificity of quinoprotein glucose dehydrogenase in Gluconobacter oxydans.
Molecular & general genetics : MGG - 1 Oct 1991
Cleton-Jansen A M, Dekker S, van de Putte P, Goosen N
Abstract excerpt
Gluconobacter oxydans contains pyrroloquinoline quinone-dependent glucose dehydrogenase (GDH). Two isogenic G. oxydans strains, P1 and P2, which differ in their substrate specificity with respect to oxidation of sugars have been analysed. P1 can oxidize only D-glucose, whereas P2 is also capable of the oxidation of the disaccharide maltose. To investigate the nature of this maltose-oxidizing property we cloned...
Topics
- Amino Acid Sequence
- Amino Acids
- Bacteriophage lambda
- Base Sequence
- Conjugation, Genetic
- Escherichia coli
- Genes, Viral
- Glucose 1-Dehydrogenase
- Glucose Dehydrogenases
- Hydrogen-Ion Concentration