Article
Frameshift mutations in the bacteriophage Mu repressor gene can confer a trans-dominant virulent phenotype to the phage.
Journal of bacteriology - 1 Oct 1991
Geuskens V, Vogel J L, Grimaud R, Desmet L, Higgins N P, Toussaint A
Abstract excerpt
Virulent mutations in the bacteriophage Mu repressor gene were isolated and characterized. Recombination and DNA sequence analysis have revealed that virulence is due to unusual frameshift mutations which change several C-terminal amino acids. The vir mutations are in the same repressor region as the sts amber mutations which, by eliminating several C-terminal amino acids, suppress thermosensitivity of repressor...
Topics
- Amino Acid Sequence
- Bacteriophage mu
- Base Sequence
- Blotting, Western
- DNA-Binding Proteins
- Frameshift Mutation
- Genes, Dominant
- Molecular Sequence Data
- Mutagenesis
- Operator Regions, Genetic